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Part:BBa_K5310027:Design

Designed by: Alexandros Liatsos   Group: iGEM24_thessaloniki   (2024-09-30)


sgRNA from FOXP3 gene for CRISPR


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Proper selection of target sequences, so as not to be overlapping. Furthermore, restriction enzyme sequences for BsmBI were also taken into account and introduced.


Source

FOXP3 gene

References